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We have recently
cloned the following new marine luciferases:
These Are Brighter & Better!
Gaussia, Pleuromamma,
and Renilla mullerei luciferases.
These NanoLight® proteins all use coelenterazine and coelenterazine
derivatives as substrate. We are currently in the process of analyzing
the activity of these new proteins. We know that the Gaussia and Renilla
proteins express well in bacteria, and that the Gaussia protein expresses
well in mammalian cells. All of these NanoLight® proteins are sodium
dependent. As more information becomes available, it will be published
here.
Nanolight Technical
Slideshow(.pdf)
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Adobe Acrobat Reader
Gaussia princeps a Copod |
Pleuromamma |
Products
for Luciferase Vector and Sequence
Please
note:
• These Vectors are sold for non-commercial research purposes
only.
• Codon
optimizations and/or mutations may not be performed without
written permission.
• Non
Commercial use means that the recipient agrees not to apply
for any patent protection for inventions containing these genes
or protein derivatives, without prior written permission from
Prolume/NanoLight.
These vectors may be purchased by Commercial (For Profit) entities under the same terms and conditions as the Tear Away Label License and used
for a free 180 day evaluation period. The use of the CMV promoter please contact University of Iowa Research Foundation . If you have any questions, please contact bruce@prolume.com
•
Click
here and read our short Tear Away Label License before placing
an order
| Catalog |
Description |
Price |
Download
Map |
| Cat#200 pUC18 GLuc |
expressing native Gaussia luciferase without the secretion signal, 25ug |
$325.00 |
download now
download now
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| Cat#201 pUC18 SS-GLuc |
expressing native Gaussia luciferase with the N-terminal secretion signal, 25ug |
$325.00 |
download now
download now
download now |
| Cat#202 pCMV-Gluc-1 |
Humanized Gaussia luciferase with secretion signal . This positive control vector is very useful in evaluating the efficiency of transgene expression using Gaussia luciferase as a reporter. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E. coli and can be used to establish stable cell lines expressing Gaussia luciferase. 25ug |
$325.00 |
download
now |
| Cat#203 pGluc-basic-1 |
A promoterless vector with a MCS site upstream of the humanized Gaussia luciferase coding sequence (with secretion signal). This vector is designed for promoter analysis and will express secreted Gaussia luciferase. 25ug |
$325.00 |
download
now |
| Cat#204 pUC19 R.M. Luc |
Native Renilla Mullerei Luciferase, 25ug |
$325.00
|
download
now |
| Cat#205 pUC-19 Ple-Luc |
Native Pleuromamma Luciferase, 25ug |
$325.00 |
download
now |
| Cat# 206 pCMV-KDEL-Gluc-1 |
Humanized Gaussia luciferase with C-terminal KDEL endoplasmic reticulum retention signal for intracellular expression. The KDEL sequence is an endoplasmic reticulum retention signal which causes retention of the Gaussia luciferase in the endoplasmic reticulum and results in high levels of intracellular luciferase expression. 25ug |
$325.00 |
download
now |
| Cat#207 pGLuc-KDEL-Basic-1 |
Humanized Gaussia Luciferase, promoterless; designed for insertion of your own promoters, comes with C-terminal KDEL endoplasmic retention signal for intracellular expression. 25ug |
$325.00 |
download
now |
| Cat#209 pCMV- Gluc minus SS |
Humanized Gaussia luciferase without secretion signal . This positive control vector is very useful in evaluating the efficiency of intracellular transgene expression using Gaussia luciferase as a reporter. Also, this vector is useful for fusing Gluc to a specific protein of interest. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E. coli and can be used to establish stable cell lines expressing Gaussia luciferase. 25ug |
$325.00 |
download
now |
|
| |
| Catalog |
Description |
Price |
Download
Protocols |
| Cat#319 GAR1 |
This reagent is used for measurement of Gaussia luciferase in applications when the luciferase activity is measured immediately after addition of the assay reagent to the luciferase containing supernatant. This reagent can be used for assaying gaussia luciferase or renilla luciferase activity in cell-supernatants or in cell lysates. This reagent is compatible for measuring luciferase activity in cells lysed with either the lysis buffer from Targeting Systems or the Promega RLAR lysis buffer or passive lysis buffers.
50 milliliters |
$350.00 |
download
now |
| Cat#320 GAR-2 |
Gaussia luciferase assay reagents for high throughput applications requiring a stable bioluminescent signal in
the first 15-30 minutes of the assay. The bioluminescent signal using this assay reagent is stable for the first 15 minutes and then declines slowly. The half life of the bioluminescent signal is 30 minutes. The intensity of the bioluminescent signal is lesser than that obtained with the GAR-1 reagent because of the effect of stabilizers in the GAR-2 reagent. 50 milliliters |
$350.00 |
download
now |
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| |
| Catalog |
Description |
Price |
Download
Protocols |
| Cat# 401 Antisera-Gluc |
Polyclonal Rabbit Antibody to Gaussia Luciferase Titer>1:10,000 250ul |
$250.00 |
download
now |
| Cat# 401A Antisera-Gluc |
Monoclonal mouse Antibody to Gaussia Luciferase available, please inquire |
$250.00 |
download
now |
| Cat#402 Antisera-Pt. GFP |
Polyclonal Rabbit Antibody to Ptilosarcus GFP Titer>1:10,000 250ul |
$250.00 |
download
now |
| Cat#403 Antisera-Rr GFP |
Polyclonal Rabbit Antibody to R.reniformis GFP Titer>1:10,000 250ul |
$250.00 |
download
now |
|
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*Reagents for Renilla
, Firefly and Gaussia luciferase are available, please visit http://www.luciferin.com
• Click
here for our commercial material transfer agreement.
PCT Patent Luciferases
•
One Page Review of
Patent
•
Download Full Version of Patent: 12.5mb(.pdf)
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