LOCUS PUC18-Gluc\with\ 3262 bp DNA circular 27-FEB-2009 DEFINITION native Gluc with signal sequence in PUC18. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|511110549| COMMENT VNTDBDATE|511111345| COMMENT LSOWNER| COMMENT VNTNAME|PUC18-Gluc with SS| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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"") COMMENT #2=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") COMMENT #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 231 0 0 "") COMMENT #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 858 0 0 "") COMMENT #5=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 850 0 0 "") COMMENT #6=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 249 0 0 "") COMMENT #7=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 247 0 0 "") COMMENT #8=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 834 0 0 "") COMMENT #9=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 247 0 0 "") COMMENT #10=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 241 0 0 "") COMMENT #11=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 840 0 0 "") COMMENT #12=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 670 0 841 0 0 "") COMMENT #13=(CRSite (CStringList) "HincII" "gtyrac" 4 0 3 596 0 659 0 842 0 0 "") COMMENT #14=(CRSite (CStringList) "SphI" "gcatgc" 6 0 1 856 0 0 "")) COMMENT (CObList COMMENT #15=(CFSignal (CObList) "native Gluc with signal sequence" 2 0 0 257 832 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "Gluc signal sequence" 94 0 0 257 307 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "6-His tag" 21 0 0 812 829 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) "AMP-R" 2 0 0 1528 2316 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #19=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #20=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) COMMENT "PUC18-Gluc with SS" 2) 5 "" 0 4) COMMENT #21=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'PUC18-Gluc with SS'" 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "native Gluc with signal sequence in PUC18." 1) COMMENT #24=(CLinePar 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ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct 241 cggtacccgg ggatccatgg gagtgaaagt tctttttgcc cttatttgta ttgctgtggc 301 cgaggccaaa ccaactgaaa acaatgaaga tttcaacatt gtagctgtag ctagcaactt 361 tgctacaacg gatctcgatg ctgaccgtgg taaattgccc ggaaaaaaat taccacttga 421 ggtactcaaa gaaatggaag ccaatgctag gaaagctggc tgcactaggg gatgtctgat 481 atgcctgtca cacatcaagt gtacacccaa aatgaagaag tttatcccag gaagatgcca 541 cacctatgaa ggagacaaag aaagtgcaca gggaggaata ggagaggcta ttgttgacat 601 tcctgaaatt cctgggttta aggatttgga acccatggaa caattcattg cacaagttga 661 cctatgtgta gactgcacaa ctggatgcct caaaggtctt gccaatgtgc aatgttctga 721 tttactcaag aaatggctgc cacaaagatg tgcaactttt gctagcaaaa ttcaaggcca 781 agtggacaaa ataaagggtg ccggtggtga tcatcaccat caccatcact aatctagagt 841 cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa 901 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 961 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 1021 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 1081 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1141 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1201 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1261 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1321 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1381 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1441 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1501 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1561 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1621 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 1681 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 1741 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 1801 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 1861 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 1921 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 1981 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2041 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2101 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2161 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2221 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2281 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2341 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2401 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2461 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2521 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2581 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 2641 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 2701 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 2761 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 2821 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 2881 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 2941 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3001 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3061 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3121 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3181 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3241 cagtgagcga ggaagcggaa ga //