LOCUS PUC18-Gluc\witho 3214 bp DNA circular 28-FEB-2009 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|511111928| COMMENT VNTDBDATE|511170233| COMMENT LSOWNER| COMMENT VNTNAME|PUC18-Gluc without SS| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList COMMENT #169=(CScratch COMMENT (CWidget 1 (1 #13# 1) 1 2 0 0 #88# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 4.741 1.9 0.082322 COMMENT 1) COMMENT #170=(CLabel (CWidget 0 (0 0) 1 2 0 0 #90# 325 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xba}I (786)" "@N (@S)" 5 14.0734 0 COMMENT -9.47137 13.3758 3.62158 0.799245 #169#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #171=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1273 100) COMMENT (CObjectList) (CObjectList)) COMMENT #172=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -65300 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers C_region 257..784 /vntifkey="2" /label=native\Gluc\without\SS /note="Native Gluc sequence" misc_signal 764..781 /vntifkey="87" /label=6-His\tag /note="6-histidine tag" C_region 1480..2268 /vntifkey="2" /label=AmpR BASE COUNT 837 a 791 c 798 g 788 t ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct 241 cggtacccgg ggatccatga aaccaactga aaacaatgaa gatttcaaca ttgtagctgt 301 agctagcaac tttgctacaa cggatctcga tgctgaccgt ggtaaattgc ccggaaaaaa 361 attaccactt gaggtactca aagaaatgga agccaatgct aggaaagctg gctgcactag 421 gggatgtctg atatgcctgt cacacatcaa gtgtacaccc aaaatgaaga agtttatccc 481 aggaagatgc cacacctatg aaggagacaa agaaagtgca cagggaggaa taggagaggc 541 tattgttgac attcctgaaa ttcctgggtt taaggatttg gaacccatgg aacaattcat 601 tgcacaagtt gacctatgtg tagactgcac aactggatgc ctcaaaggtc ttgccaatgt 661 gcaatgttct gatttactca agaaatggct gccacaaaga tgtgcaactt ttgctagcaa 721 aattcaaggc caagtggaca aaataaaggg tgccggtggt gatcatcacc atcaccatca 781 ctaatctaga gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc 841 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg 901 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 961 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 1021 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 1081 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 1141 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 1201 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 1261 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 1321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 1381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 1441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 1501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 1561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 1621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 1681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 1741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 1801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 1861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 1921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 1981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 2041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 2101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 2161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 2221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 2281 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 2341 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 2401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 2461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 2521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 2581 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 2641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 2701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 2761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 2821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 2881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 2941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 3061 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 3121 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 3181 agcgcagcga gtcagtgagc gaggaagcgg aaga //